This is the process by which glucose is synthesized from lactate and pyruvate. It occurs mainly in the liver and kidneys. This animated presentation offers an introduction to glucose energy, its regulation and its synthesis. Then we look at the energetics: free-energy changes, large changes and barriers. The "Pathway" section takes us step by step through the process of converting pyruvate to phosphoenolpyruvate. In the "Control" section we learn about the points at which gluconeogenesis is regulated. In the "Interactive" section we have to place the correct substrate into its correct position in the gluconeogenesis pathway.